You state you are "Considering putting 2x6 joists over a 7 inch thick concrete slab. Over that 3/4 plywood and 3/4 inch T&G hardwood flooring."
What is this space being used for?
How are you climate controlling the space?
It would be easier to help with a solution if we had more detail on the intended use & buildout of the space.
I will be upfront & say as a contractor I don't have much experience with Perlite except as as a concrete block insulation filler.... (& it's use in soilless potting mixes). So your application intrigues me & I hope to hear from someone with better knowledge on the subject.
I due know Perlite insulation materials do not support combustion, are resistant to mold, rot, and rodents so I would not rule them out; I personally do not have enough experience with it to advise on it use in this application. We use Roxul (rockwool) insulation & closed cell spray foam for may of our projects; so I hope someone with more Perlite experience steps in.... (I love to learn)
From a quick search Perlite has an approx. R-value of 2.7 per inch.... the Closed cell foam we use yields a R-value of 6.5 to 7 per inch & provides a air tight vapor barrier.
Either way my first step would be sealing the existing 25yr old slab & for that I would look at using Sanitred (or similar product), we have been using Sanitred for 18+ yrs with no failures (if prepped & applied properly) bonus it is a radon barrier also.
Then if this is going to be living space I might consider installing hydronic radiant floor heating below the flooring (we are in New England) but should work equally as well in Ohio. If I was doing that I probably would look at Closed Cell Spray Foam as my first choice.... Again a lot has to do with the intended use of the space &the rest of the construction practices to the structure.
I really hope there is someone with Perlite real world experience that can give you better info....