From this it didn't work out as well as expected,
History
In 2013, reported initial experiments to introduce wheat OxO into American chestnuts. Potted transgenic plants with two different
promoters (35S, VspB) were created. OxO levels are measured out of the plant leaves. Infection experiments on cut leaves show that the lesion sizes can be reduced to around or below the level of the blight-resistant
Chinese chestnut, suggesting that the potted plant may be resistant too.
[11][12]
In 2014, SUNY ESF reported that the "Darling4" transgenic event produced an intermediate level of resistance between American and Chinese chestnuts. The trait was also passed into progeny.
[8][13]
The Darling 58 (SX58) line was produced before 2016. A 2020 SUNY-ESF Masters thesis shows that Darling 58 is the transgenic event that produces the highest amount of OxO.
[7]
In January 2020, the researchers submitted a deregulation petition for the Darling 58 variant, with a public comment period ending October 19, 2020.
[14][15]
In November 2022, the USDA began another public comment period for Darling 58's approval.
[16]
In 2022, SUNY-ESF scientists reported that a different promoter, win3.12 from the
eastern cottonwood, allows the expression levels of OxO to remain low in basal conditions, but increase under wound or infection. This modification is expected to be more metabolically efficient compared to the "always-on" CAMV promoter and thereby have greater transgene stability over successive generations compared with the Darling 58 variant. In laboratory bioassays, win3.12-OxO lines showed elevated disease tolerance similar to that exhibited by blight-resistant Chinese chestnut.
[17]
In December 2023, the TACF announced that they were discontinuing development of the Darling 58 due to poor performance results.
[18] The SUNY ESF is continuing to seek federal approval to distribute seeds to the public without the support of the TACF.
[19]
This is from here; https://en.wikipedia.org/wiki/Darling_58
And from the American Chestnut Foundation; why darling 58 has failed
Darling 58 | The American Chestnut Foundation